Journal: Animals : an Open Access Journal from MDPI
Article Title: Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine
doi: 10.3390/ani10091549
Figure Lengend Snippet: The role of proline dehydrogenase in the antioxidant properties of proline. ( A ) Effect of proline on the activity of PRODH on day 5 of storage. ( B ) Effect of inhibition of proline dehydrogenase on the progressive motility of sperm during H 2 O exposure. Samples were treated with or without H 2 O 2 in the presence of proline or THFA and incubated at 37 °C for 2 h. Columns with nonidentical superscripts are significantly ( p < 0.05) different from each other in statistics.
Article Snippet: A proline dehydrogenase (PRODH) assay kit (BC4165, Beijing solarbio science & technology Co., Ltd., Beijing, China) was used to detect the activity of PRODH in boar sperm according to the manufacturer’s instruction.
Techniques: Activity Assay, Inhibition, Incubation